CA2287730A1 - Method for purification and manipulation of nucleic acids using paramagentic particles - Google Patents
Method for purification and manipulation of nucleic acids using paramagentic particles Download PDFInfo
- Publication number
- CA2287730A1 CA2287730A1 CA002287730A CA2287730A CA2287730A1 CA 2287730 A1 CA2287730 A1 CA 2287730A1 CA 002287730 A CA002287730 A CA 002287730A CA 2287730 A CA2287730 A CA 2287730A CA 2287730 A1 CA2287730 A1 CA 2287730A1
- Authority
- CA
- Canada
- Prior art keywords
- paramagentic
- manipulation
- purification
- particles
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The present invention relates to a composition which is useful for the reversible binding of a nucleic acid molecule. The composition, which may be packaged in a kit, includes a paramagnetic particle in an acidic solution.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/183,127 US5973138A (en) | 1998-10-30 | 1998-10-30 | Method for purification and manipulation of nucleic acids using paramagnetic particles |
US09/183,127 | 1998-10-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2287730A1 true CA2287730A1 (en) | 2000-04-30 |
CA2287730C CA2287730C (en) | 2008-12-23 |
Family
ID=22671554
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002287730A Expired - Lifetime CA2287730C (en) | 1998-10-30 | 1999-10-28 | Method for purification and manipulation of nucleic acids using paramagentic particles |
Country Status (7)
Country | Link |
---|---|
US (2) | US5973138A (en) |
EP (2) | EP1002860B1 (en) |
JP (1) | JP4594467B2 (en) |
AU (1) | AU771249B2 (en) |
CA (1) | CA2287730C (en) |
DE (2) | DE69942639D1 (en) |
ES (2) | ES2349717T3 (en) |
Families Citing this family (94)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6884357B2 (en) | 1995-02-21 | 2005-04-26 | Iqbal Waheed Siddiqi | Apparatus and method for processing magnetic particles |
US6048734A (en) | 1995-09-15 | 2000-04-11 | The Regents Of The University Of Michigan | Thermal microvalves in a fluid flow method |
US6872527B2 (en) * | 1997-04-16 | 2005-03-29 | Xtrana, Inc. | Nucleic acid archiving |
JP2002502856A (en) * | 1998-02-04 | 2002-01-29 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング | Methods for isolating and purifying nucleic acids |
JP4457425B2 (en) * | 1998-09-08 | 2010-04-28 | アイシン精機株式会社 | Motor armature core and brushed DC motor using the armature core |
US5973138A (en) * | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
US20040005673A1 (en) | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
US6936414B2 (en) * | 1999-12-22 | 2005-08-30 | Abbott Laboratories | Nucleic acid isolation method and kit |
ES2319101T3 (en) * | 2000-05-19 | 2009-05-04 | Becton Dickinson And Company | SYSTEM AND PROCEDURE FOR MAGNETICALLY SENSITIVE PARTICLE HANDLING IN FLUID SAMPLES TO EXTRACT DNA OR RNA FROM A SAMPLE. |
US6672458B2 (en) * | 2000-05-19 | 2004-01-06 | Becton, Dickinson And Company | System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample |
US20050272085A1 (en) * | 2000-09-06 | 2005-12-08 | Hodge Timothy A | Methods for forensic and congenic screening |
US20050239125A1 (en) * | 2000-09-06 | 2005-10-27 | Hodge Timothy A | Methods for genotype screening |
US20050266494A1 (en) * | 2000-09-06 | 2005-12-01 | Hodge Timothy A | System and method for computer network ordering of biological testing |
US7494817B2 (en) * | 2000-09-06 | 2009-02-24 | Transnet Yx, Inc. | Methods for genotype screening using magnetic particles |
EP1978110B1 (en) * | 2000-09-06 | 2010-05-26 | Transnetyx, Inc. | Computer-based method and system for screening genomic DNA |
US6692700B2 (en) | 2001-02-14 | 2004-02-17 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
US6852287B2 (en) | 2001-09-12 | 2005-02-08 | Handylab, Inc. | Microfluidic devices having a reduced number of input and output connections |
US7010391B2 (en) | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
US7323140B2 (en) | 2001-03-28 | 2008-01-29 | Handylab, Inc. | Moving microdroplets in a microfluidic device |
US20030092029A1 (en) * | 2001-06-06 | 2003-05-15 | Lee Josephson | Magneitc-nanoparticle conjugates and methods of use |
US20030040129A1 (en) * | 2001-08-20 | 2003-02-27 | Shah Haresh P. | Binding assays using magnetically immobilized arrays |
KR20050008720A (en) | 2002-05-17 | 2005-01-21 | 벡톤 디킨슨 앤드 컴퍼니 | Automated system for isolating, amplifying and detecting a target nucleic acid sequence |
US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
EP1654066B1 (en) | 2003-07-31 | 2014-11-12 | Handylab, Inc. | Processing particle-containing samples |
NZ545469A (en) * | 2003-09-12 | 2009-11-27 | Biocontrol Systems Inc | Methods, compositions, and kits for the concentration and detection of microorganisms without a separate DNA extraction step |
US20080305048A1 (en) * | 2003-12-10 | 2008-12-11 | The General Hospital Corporation | Self-Assembling Nanoparticle Conjugates |
DE10358137A1 (en) * | 2003-12-12 | 2005-07-07 | Merck Patent Gmbh | Method and kit for isolating RNA |
WO2005093065A1 (en) * | 2004-03-16 | 2005-10-06 | Roche Diagnostics Gmbh | Improved method of isolating nucleic acids |
CA2502549C (en) | 2004-04-23 | 2016-02-16 | Becton, Dickinson And Company | Use of an extraction control in a method of extracting nucleic acids |
US20050239091A1 (en) * | 2004-04-23 | 2005-10-27 | Collis Matthew P | Extraction of nucleic acids using small diameter magnetically-responsive particles |
US8852862B2 (en) * | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
AU2005241080B2 (en) | 2004-05-03 | 2011-08-11 | Handylab, Inc. | Processing polynucleotide-containing samples |
US9267167B2 (en) | 2004-06-28 | 2016-02-23 | Becton, Dickinson And Company | Dissolvable films and methods including the same |
JP4476050B2 (en) * | 2004-06-30 | 2010-06-09 | 株式会社ニデック | Perimeter |
US20060024776A1 (en) * | 2004-08-02 | 2006-02-02 | Mcmillian Ray | Magnetic particle capture of whole intact organisms from clinical samples |
US8615368B2 (en) * | 2005-03-10 | 2013-12-24 | Gen-Probe Incorporated | Method for determining the amount of an analyte in a sample |
US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
AU2006284832B2 (en) | 2005-08-31 | 2011-06-02 | T2 Biosystems Inc. | NMR device for detection of analytes involving magnetic particles |
US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
WO2007112114A2 (en) | 2006-03-24 | 2007-10-04 | Handylab, Inc. | Integrated system for processing microfluidic samples, and method of using same |
US8883490B2 (en) | 2006-03-24 | 2014-11-11 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
WO2008061165A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic cartridge and method of making same |
CA2692186C (en) * | 2007-06-29 | 2019-03-12 | Becton, Dickinson And Company | Methods for extraction and purification of components of biological samples |
US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
US20090136385A1 (en) | 2007-07-13 | 2009-05-28 | Handylab, Inc. | Reagent Tube |
US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US8324372B2 (en) | 2007-07-13 | 2012-12-04 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
US8133671B2 (en) | 2007-07-13 | 2012-03-13 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
USD621060S1 (en) | 2008-07-14 | 2010-08-03 | Handylab, Inc. | Microfluidic cartridge |
US9097644B2 (en) * | 2007-08-17 | 2015-08-04 | Massachusetts Institute Of Technology | Magnetic resonance-based viscometers and methods |
US20090088560A1 (en) * | 2007-10-02 | 2009-04-02 | Hong Shen | Process for Nucleic Acid Purification |
US20100041034A1 (en) * | 2008-04-14 | 2010-02-18 | Murante Richard S | Method for manipulating samples with magnetic nucleation nanoparticles |
USD618820S1 (en) | 2008-07-11 | 2010-06-29 | Handylab, Inc. | Reagent holder |
USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
ES2567067T3 (en) * | 2008-11-28 | 2016-04-19 | F. Hoffmann-La Roche Ag | System and method for automatic nucleic acid extraction |
EP2191900B1 (en) * | 2008-11-28 | 2016-03-30 | F. Hoffmann-La Roche AG | System and method for nucleic acids containing fluid processing |
CN113145297B (en) | 2009-05-15 | 2023-09-29 | 简·探针公司 | Method and apparatus for automatic movement of magnet in instrument for performing magnetic separation process |
US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
US8222397B2 (en) | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
EP2473595A4 (en) | 2009-08-31 | 2013-04-24 | Mbio Diagnostics Inc | Integrated sample preparation and analyte detection |
CA2793144A1 (en) * | 2010-03-16 | 2011-09-22 | Danielle Hilligoss | Use of achromopeptidase for lysis at room temperature |
DK2611916T3 (en) * | 2010-09-02 | 2017-04-10 | Qiagen Gmbh | PROCEDURE FOR INSULATING A TARGET NUCLEIC ACID, INCLUDING SMALL TARGET ACIDS, WITH HIGH Yield |
EP2630517B1 (en) | 2010-10-22 | 2016-03-16 | T2 Biosystems, Inc. | Nmr systems and methods for the rapid detection of analytes |
US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
US8409807B2 (en) | 2010-10-22 | 2013-04-02 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
WO2012142516A1 (en) | 2011-04-15 | 2012-10-18 | Becton, Dickinson And Company | Scanning real-time microfluidic thermo-cycler and methods for synchronized thermocycling and scanning optical detection |
AU2012315595B2 (en) | 2011-09-30 | 2015-10-22 | Becton, Dickinson And Company | Unitized reagent strip |
USD692162S1 (en) | 2011-09-30 | 2013-10-22 | Becton, Dickinson And Company | Single piece reagent holder |
CN104040238B (en) | 2011-11-04 | 2017-06-27 | 汉迪拉布公司 | Polynucleotides sample preparation apparatus |
EP2795289B1 (en) | 2011-12-21 | 2022-08-31 | Becton Dickinson and Company | Flow cytometric systems for sterile separation of magnetically labeled sample components |
BR112014018995B1 (en) | 2012-02-03 | 2021-01-19 | Becton, Dickson And Company | systems to perform automated testing |
EP2839038B1 (en) | 2012-04-20 | 2019-01-16 | T2 Biosystems, Inc. | Compositions and methods for detection of candida species |
US20140322706A1 (en) | 2012-10-24 | 2014-10-30 | Jon Faiz Kayyem | Integrated multipelx target analysis |
EP2912432B1 (en) | 2012-10-24 | 2018-07-04 | Genmark Diagnostics Inc. | Integrated multiplex target analysis |
AU2013202805B2 (en) | 2013-03-14 | 2015-07-16 | Gen-Probe Incorporated | System and method for extending the capabilities of a diagnostic analyzer |
WO2014150905A2 (en) | 2013-03-15 | 2014-09-25 | Genmark Diagnostics, Inc. | Systems, methods, and apparatus for manipulating deformable fluid vessels |
USD881409S1 (en) | 2013-10-24 | 2020-04-14 | Genmark Diagnostics, Inc. | Biochip cartridge |
US9498778B2 (en) | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
JP2017539080A (en) | 2014-10-23 | 2017-12-28 | コーニング インコーポレイテッド | Polymer encapsulated magnetic nanoparticles |
US10005080B2 (en) | 2014-11-11 | 2018-06-26 | Genmark Diagnostics, Inc. | Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation |
US9598722B2 (en) | 2014-11-11 | 2017-03-21 | Genmark Diagnostics, Inc. | Cartridge for performing assays in a closed sample preparation and reaction system |
CN107257857B (en) | 2014-11-14 | 2021-12-14 | 康宁股份有限公司 | Methods and kits for post-IVT RNA purification |
FR3031063B1 (en) | 2014-12-30 | 2017-02-10 | Biomerieux Sa | MULTILAYER COMPLEX, PROCESS FOR MANUFACTURING AND USE OF THE COMPLEX |
US11519016B2 (en) | 2016-01-21 | 2022-12-06 | T2 Biosystems, Inc. | NMR methods and systems for the rapid detection of bacteria |
DE112019004459T5 (en) | 2018-09-05 | 2021-07-15 | Adana Bilim Ve Teknoloji Universitesi | ENRICHMENT OF SAMPLES BY MEANS OF MAGNETIC PARTICLES IN MICROCHANNELS |
Family Cites Families (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US280130A (en) | 1883-06-26 | Method of and apparatus for separating | ||
US3985649A (en) | 1974-11-25 | 1976-10-12 | Eddelman Roy T | Ferromagnetic separation process and material |
US4018886A (en) | 1975-07-01 | 1977-04-19 | General Electric Company | Diagnostic method and device employing protein-coated magnetic particles |
US3970518A (en) * | 1975-07-01 | 1976-07-20 | General Electric Company | Magnetic separation of biological particles |
GB1575805A (en) * | 1976-03-12 | 1980-10-01 | Technicon Instr | Automatic diagnostic apparatus |
US4272510A (en) | 1976-04-26 | 1981-06-09 | Smith Kendall O | Magnetic attraction transfer process for use in solid phase radioimmunoassays and in other assay methods |
US4230685A (en) * | 1979-02-28 | 1980-10-28 | Northwestern University | Method of magnetic separation of cells and the like, and microspheres for use therein |
EP0030086B2 (en) | 1979-11-13 | 1990-03-14 | TECHNICON INSTRUMENTS CORPORATION (a New York corporation) | Test-tube assembly, kit for making it and method of manual immunoassay |
NO155316C (en) * | 1982-04-23 | 1987-03-11 | Sintef | PROCEDURE FOR MAKING MAGNETIC POLYMER PARTICLES. |
US4672040A (en) * | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
US4695393A (en) * | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
CH663476A5 (en) | 1985-07-08 | 1987-12-15 | Serono Diagnostics Ltd | ENCLOSURE FOR THE DETERMINATION OF ANTIBODIES OR ANTIGENS IN A BIOLOGICAL LIQUID. |
US5076950A (en) * | 1985-12-20 | 1991-12-31 | Syntex (U.S.A.) Inc. | Magnetic composition for particle separation |
US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
CA1297431C (en) * | 1987-04-24 | 1992-03-17 | F. Hoffmann-La Roche Ag | Process for the isolation of nucleic acids |
US5395688A (en) * | 1987-10-26 | 1995-03-07 | Baxter Diagnostics Inc. | Magnetically responsive fluorescent polymer particles |
US4923978A (en) * | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
GB8827160D0 (en) * | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
DE68913555T2 (en) * | 1988-11-21 | 1994-07-07 | Dynal As | NUCLEIC ACID PROBE. |
US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
US5279936A (en) * | 1989-12-22 | 1994-01-18 | Syntex (U.S.A.) Inc. | Method of separation employing magnetic particles and second medium |
FR2656318B1 (en) * | 1989-12-27 | 1994-02-04 | Rhone Poulenc Chimie | MAGNETISABLE CORE-SHELL MICROSPHERES BASED ON CROSSLINKED ORGANOPOLYSILOXANE, THEIR PREPARATION PROCESS AND THEIR APPLICATION IN BIOLOGY. |
US5523231A (en) * | 1990-02-13 | 1996-06-04 | Amersham International Plc | Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules |
US5129936A (en) * | 1990-07-30 | 1992-07-14 | Wilson Harold W | Processes for the preparation of acid fortified paramagnetic iron sulfate salt compounds for use in the treatment of agricultural soils |
US5491068A (en) * | 1991-02-14 | 1996-02-13 | Vicam, L.P. | Assay method for detecting the presence of bacteria |
DE69329135T2 (en) * | 1992-09-24 | 2001-01-11 | Amersham Pharm Biotech Uk Ltd | Magnetic deposition method and apparatus |
FI932866A0 (en) * | 1993-06-21 | 1993-06-21 | Labsystems Oy | Separeringsfoerfarande |
US5916539A (en) * | 1993-03-02 | 1999-06-29 | Silica Gel Ges. M.B.H. | Superparamagnetic particles, process for producing the same and their use |
GB9323305D0 (en) | 1993-11-11 | 1994-01-05 | Medinnova Sf | Isoaltion of nucleic acid |
US5602042A (en) | 1994-04-14 | 1997-02-11 | Cytyc Corporation | Method and apparatus for magnetically separating biological particles from a mixture |
DE59508069D1 (en) | 1994-07-27 | 2000-04-27 | Herbert Pilgrimm | SUPERPARAMAGNETIC PARTICLES, METHOD FOR THE PRODUCTION AND USE THEREOF |
US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
FR2732116B1 (en) | 1995-03-21 | 1997-05-09 | Bio Merieux | METHOD AND DEVICE FOR THE QUALITATIVE AND / OR QUANTITATIVE DETERMINATION OF AN ANALYTE, IN PARTICULAR OF A BACTERIA, IN A SAMPLE, BY MAGNETIC WAY |
DE19520398B4 (en) * | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetic pigment |
JP3940935B2 (en) * | 1996-06-11 | 2007-07-04 | 東洋紡績株式会社 | Extraction and purification method of plasmid DNA |
JPH09327291A (en) * | 1996-06-11 | 1997-12-22 | Toyobo Co Ltd | Extraction and purification of rna |
DE69734263T2 (en) * | 1996-07-12 | 2006-07-13 | Toyo Boseki K.K. | Process for isolating ribonucleic acids. |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
US5973138A (en) * | 1998-10-30 | 1999-10-26 | Becton Dickinson And Company | Method for purification and manipulation of nucleic acids using paramagnetic particles |
-
1998
- 1998-10-30 US US09/183,127 patent/US5973138A/en not_active Expired - Lifetime
-
1999
- 1999-09-20 EP EP99118529A patent/EP1002860B1/en not_active Expired - Lifetime
- 1999-09-20 DE DE69942639T patent/DE69942639D1/en not_active Expired - Lifetime
- 1999-09-20 ES ES05112446T patent/ES2349717T3/en not_active Expired - Lifetime
- 1999-09-20 DE DE69933186T patent/DE69933186T2/en not_active Expired - Lifetime
- 1999-09-20 ES ES99118529T patent/ES2270556T3/en not_active Expired - Lifetime
- 1999-09-20 EP EP05112446A patent/EP1674571B1/en not_active Expired - Lifetime
- 1999-10-08 US US09/417,199 patent/US6433160B1/en not_active Expired - Lifetime
- 1999-10-22 JP JP30045299A patent/JP4594467B2/en not_active Expired - Lifetime
- 1999-10-27 AU AU57073/99A patent/AU771249B2/en not_active Expired
- 1999-10-28 CA CA002287730A patent/CA2287730C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2287730C (en) | 2008-12-23 |
EP1674571A3 (en) | 2008-08-13 |
US5973138A (en) | 1999-10-26 |
JP4594467B2 (en) | 2010-12-08 |
EP1674571A2 (en) | 2006-06-28 |
EP1002860B1 (en) | 2006-09-13 |
US6433160B1 (en) | 2002-08-13 |
AU771249B2 (en) | 2004-03-18 |
AU5707399A (en) | 2000-06-01 |
DE69942639D1 (en) | 2010-09-09 |
DE69933186D1 (en) | 2006-10-26 |
ES2349717T3 (en) | 2011-01-10 |
ES2270556T3 (en) | 2007-04-01 |
JP2000159791A (en) | 2000-06-13 |
EP1674571B1 (en) | 2010-07-28 |
DE69933186T2 (en) | 2007-09-20 |
EP1002860A1 (en) | 2000-05-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2287730A1 (en) | Method for purification and manipulation of nucleic acids using paramagentic particles | |
AU8909998A (en) | Methods and compositions for cloning nucleic acid molecules | |
HUP9901321A3 (en) | Methods and compositions for determining the sequence of nucleic acid molecules | |
AU2001255518A1 (en) | Compositions and methods for array-based nucleic acid hybridization | |
CA2352490A1 (en) | Magnetic particles for purifying nucleic acids | |
EP0391608A3 (en) | Metal oxide supports for nucleic acids | |
EP0981511A4 (en) | Methods and compositions for the selective modification of nucleic acids | |
CA2273078A1 (en) | Use of nucleic acids bound to carrier macromolecules | |
AU4145699A (en) | Modified gellan gum composition process for preparation of same and use thereof | |
EP1679383A3 (en) | Method and device for purifying nucleid acids | |
CA2343130A1 (en) | Cosmetic composition | |
EP1141234A4 (en) | Methods and compositions for isolation of nucleic acid molecules | |
EP1339875A4 (en) | Compositions and methods for rapidly generating recombinant nucleic acid molecules | |
AU2773695A (en) | Compositions for and methods of enhancing delivery of nucleic acids to cells | |
EP0897978A3 (en) | Zirconium oxide and related compounds for purification of nucleic acids | |
DE4311039B4 (en) | Waterborne binder for aqueous nail polishes and process for its preparation | |
AU2860401A (en) | Compositions comprising nucleic acids incorporated in bilaminar mineral particles | |
AU2003233717A1 (en) | Methods and compositions for treating neoplasia relating to hnrnp a1 and a2 nucleic acid molecules | |
AU2001276023A1 (en) | Materials and methods for binding nucleic acids to surfaces | |
AU8505898A (en) | Nucleic acid compositions and methods of introducing nucleic acids into cells | |
PL342045A1 (en) | 2,3,4,5-tetrahydro-1h-[1,4]-benzdiazepin-3-hydroxamic acids as inhibitors of matrix metaloproteinases | |
AU3161401A (en) | Methods for the preparation and use of internal standards for nucleic acid amplification assays | |
AU2001270691A1 (en) | Compositions and methods for treating or detecting degenerative diseases of the motor neurons | |
DE59806159D1 (en) | Process for the preparation of 2-carboxyy-5-nitro-benzenesulfonic acid and its salts by oxidation | |
AU2001260313A1 (en) | Compositions and methods for nucleic acid analyses |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20191028 |