CA2287730A1 - Method for purification and manipulation of nucleic acids using paramagentic particles - Google Patents

Method for purification and manipulation of nucleic acids using paramagentic particles Download PDF

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Publication number
CA2287730A1
CA2287730A1 CA002287730A CA2287730A CA2287730A1 CA 2287730 A1 CA2287730 A1 CA 2287730A1 CA 002287730 A CA002287730 A CA 002287730A CA 2287730 A CA2287730 A CA 2287730A CA 2287730 A1 CA2287730 A1 CA 2287730A1
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CA
Canada
Prior art keywords
paramagentic
manipulation
purification
particles
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002287730A
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French (fr)
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CA2287730C (en
Inventor
Matthew P. Collis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
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Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of CA2287730A1 publication Critical patent/CA2287730A1/en
Application granted granted Critical
Publication of CA2287730C publication Critical patent/CA2287730C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention relates to a composition which is useful for the reversible binding of a nucleic acid molecule. The composition, which may be packaged in a kit, includes a paramagnetic particle in an acidic solution.
CA002287730A 1998-10-30 1999-10-28 Method for purification and manipulation of nucleic acids using paramagentic particles Expired - Lifetime CA2287730C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/183,127 US5973138A (en) 1998-10-30 1998-10-30 Method for purification and manipulation of nucleic acids using paramagnetic particles
US09/183,127 1998-10-30

Publications (2)

Publication Number Publication Date
CA2287730A1 true CA2287730A1 (en) 2000-04-30
CA2287730C CA2287730C (en) 2008-12-23

Family

ID=22671554

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002287730A Expired - Lifetime CA2287730C (en) 1998-10-30 1999-10-28 Method for purification and manipulation of nucleic acids using paramagentic particles

Country Status (7)

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US (2) US5973138A (en)
EP (2) EP1002860B1 (en)
JP (1) JP4594467B2 (en)
AU (1) AU771249B2 (en)
CA (1) CA2287730C (en)
DE (2) DE69942639D1 (en)
ES (2) ES2349717T3 (en)

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Also Published As

Publication number Publication date
CA2287730C (en) 2008-12-23
EP1674571A3 (en) 2008-08-13
US5973138A (en) 1999-10-26
JP4594467B2 (en) 2010-12-08
EP1674571A2 (en) 2006-06-28
EP1002860B1 (en) 2006-09-13
US6433160B1 (en) 2002-08-13
AU771249B2 (en) 2004-03-18
AU5707399A (en) 2000-06-01
DE69942639D1 (en) 2010-09-09
DE69933186D1 (en) 2006-10-26
ES2349717T3 (en) 2011-01-10
ES2270556T3 (en) 2007-04-01
JP2000159791A (en) 2000-06-13
EP1674571B1 (en) 2010-07-28
DE69933186T2 (en) 2007-09-20
EP1002860A1 (en) 2000-05-24

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