DE69933186D1 - Methode zur Reinigung und Manipulation von Nukleinsäure mittels paramagnetischen Partikeln - Google Patents

Methode zur Reinigung und Manipulation von Nukleinsäure mittels paramagnetischen Partikeln

Info

Publication number
DE69933186D1
DE69933186D1 DE69933186T DE69933186T DE69933186D1 DE 69933186 D1 DE69933186 D1 DE 69933186D1 DE 69933186 T DE69933186 T DE 69933186T DE 69933186 T DE69933186 T DE 69933186T DE 69933186 D1 DE69933186 D1 DE 69933186D1
Authority
DE
Germany
Prior art keywords
manipulation
purification
nucleic acid
paramagnetic particles
paramagnetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69933186T
Other languages
English (en)
Other versions
DE69933186T2 (de
Inventor
Matthew P Collis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of DE69933186D1 publication Critical patent/DE69933186D1/de
Publication of DE69933186T2 publication Critical patent/DE69933186T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
DE69933186T 1998-10-30 1999-09-20 Methode zur Reinigung und Manipulation von Nukleinsäure mittels paramagnetischen Partikeln Expired - Lifetime DE69933186T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/183,127 US5973138A (en) 1998-10-30 1998-10-30 Method for purification and manipulation of nucleic acids using paramagnetic particles
US183127 1998-10-30

Publications (2)

Publication Number Publication Date
DE69933186D1 true DE69933186D1 (de) 2006-10-26
DE69933186T2 DE69933186T2 (de) 2007-09-20

Family

ID=22671554

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69942639T Expired - Lifetime DE69942639D1 (de) 1998-10-30 1999-09-20 Verwendung von paramagnetischen Werkstoff zur Reinigung und Manipulation von Nukleinsäuren
DE69933186T Expired - Lifetime DE69933186T2 (de) 1998-10-30 1999-09-20 Methode zur Reinigung und Manipulation von Nukleinsäure mittels paramagnetischen Partikeln

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69942639T Expired - Lifetime DE69942639D1 (de) 1998-10-30 1999-09-20 Verwendung von paramagnetischen Werkstoff zur Reinigung und Manipulation von Nukleinsäuren

Country Status (7)

Country Link
US (2) US5973138A (de)
EP (2) EP1002860B1 (de)
JP (1) JP4594467B2 (de)
AU (1) AU771249B2 (de)
CA (1) CA2287730C (de)
DE (2) DE69942639D1 (de)
ES (2) ES2349717T3 (de)

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Also Published As

Publication number Publication date
EP1674571B1 (de) 2010-07-28
EP1002860B1 (de) 2006-09-13
US5973138A (en) 1999-10-26
ES2349717T3 (es) 2011-01-10
DE69933186T2 (de) 2007-09-20
EP1674571A3 (de) 2008-08-13
CA2287730A1 (en) 2000-04-30
AU5707399A (en) 2000-06-01
EP1674571A2 (de) 2006-06-28
CA2287730C (en) 2008-12-23
US6433160B1 (en) 2002-08-13
JP2000159791A (ja) 2000-06-13
AU771249B2 (en) 2004-03-18
EP1002860A1 (de) 2000-05-24
ES2270556T3 (es) 2007-04-01
JP4594467B2 (ja) 2010-12-08
DE69942639D1 (de) 2010-09-09

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